Hmm, I tried around a week ago for the portal 2 cards and had no issues then and I just tried right now and it seems to work fine
Not really sure what to say but have you tried verifying game cache? There is also, although I hate to say it, reinstalling it. My portal2 that works is one I just downloaded right before I got the cards. Aside from that, not really sure what else there is other than updating gpu or other drivers
Edit:
I am seeing others complaining they are having issues with portal2 crashing. So it might be possible that there is no fix for it right now :S BUT one guy did say that verifying cache worked for him (but he is the only case of the half dozen I see that worked).
sources:
http://steamcommunity.com/app/620/discussions/0/810925580037614464/
http://steamcommunity.com/app/620/discussions/0/846954921820527863/
http://steamcommunity.com/app/620/discussions/0/846954921892490598/
Edit2:
Okay, there are much more than I thought; further searching has many people having the same issue and no-one seems to know any direct reason to its cause.